Morpholinos to make mice dystrophic then fix them
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Morpholinos to make mice dystrophic then fix them
Jon Moulton Thu Mar 25, 2010 11:31 am
Fletcher S, Adams AM, Johnsen RD, Greer K, Moulton HM, Wilton SD. Dystrophin Isoform Induction In Vivo by Antisense-mediated Alternative Splicing. Mol Ther. 2010 Mar 23. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/20332768
No animal model for a mutation? Wilton et al. use one Morpholino to alter splicing and phenocopy a mutation at the RNA level, then another Morpholino to remove another exon and restore the reading frame. The resulting protein has the same structure as one produced by a mutant lacking an exon that is treated with a frame-correcting oligo.
http://www.ncbi.nlm.nih.gov/pubmed/20332768
No animal model for a mutation? Wilton et al. use one Morpholino to alter splicing and phenocopy a mutation at the RNA level, then another Morpholino to remove another exon and restore the reading frame. The resulting protein has the same structure as one produced by a mutant lacking an exon that is treated with a frame-correcting oligo.
Jon Moulton- Posts : 5721
Join date : 2010-03-12
Age : 61
Location : Philomath, Oregon, USA -
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